Absorption, conjugation and excretion of the flavanones, naringenin and hesperetin from alpha-rhamnosidase-treated orange juice in human subjects.
Identifieur interne : 001B16 ( Main/Exploration ); précédent : 001B15; suivant : 001B17Absorption, conjugation and excretion of the flavanones, naringenin and hesperetin from alpha-rhamnosidase-treated orange juice in human subjects.
Auteurs : Lea Bredsdorff [Danemark] ; Inge Lise F. Nielsen ; Salka E. Rasmussen ; Claus Cornett ; Denis Barron ; Florilene Bouisset ; Elizabeth Offord ; Gary WilliamsonSource :
- The British journal of nutrition [ 1475-2662 ] ; 2010.
English descriptors
- KwdEn :
- Beverages (analysis), Biological Availability, Citrus sinensis, Cross-Over Studies, Disaccharides (pharmacokinetics), Double-Blind Method, Female, Flavanones (analysis), Flavanones (metabolism), Flavanones (pharmacokinetics), Fruit (chemistry), Glucosides (analysis), Glucosides (metabolism), Glucosides (pharmacokinetics), Glycoside Hydrolases (administration & dosage), Glycoside Hydrolases (metabolism), Hesperidin (metabolism), Hesperidin (pharmacokinetics), Humans, Male, Placebos.
- MESH :
- chemical , administration & dosage : Glycoside Hydrolases.
- chemical , analysis : Flavanones, Glucosides.
- chemical , metabolism : Flavanones, Glucosides, Glycoside Hydrolases, Hesperidin.
- chemical , pharmacokinetics : Disaccharides, Flavanones, Glucosides, Hesperidin.
- analysis : Beverages.
- chemistry : Fruit.
- Biological Availability, Citrus sinensis, Cross-Over Studies, Double-Blind Method, Female, Humans, Male, Placebos.
Abstract
We have determined the absorption, conjugation and excretion of naringenin-7-O-rutinoside (narirutin) compared to the corresponding glucoside in an orange juice matrix in human subjects. Healthy volunteers (eight men and eight women), in a double blind, randomised, crossover study, consumed orange juice with (1) natural content of naringenin-7-O-rutinoside; (2) alpha-rhamnosidase-treated to yield naringenin-7-O-glucoside. Blood was sampled at twelve time points and three fractions of urine were collected over 24 h. The area under the plasma-time curve of naringenin from (2) alpha-rhamnosidase-treated orange juice was increased about 4-fold (P < 0.0001), peak plasma concentration (Cmax) was 5.4-fold higher (P < 0.0001) and Tmax was decreased from 311 to 92 min (P = 0.002) compared to untreated orange juice (1), indicating a change in absorption site from the colon to the small intestine. Furthermore, the amount in urine was increased from 7 to 47 % (P < 0.0001) of the dose after consumption of the alpha-rhamnosidase-treated orange juice (2). All urine samples contained both naringenin-7- and -4'-O-glucuronides. In addition, to examine the effect of dose and alpha-rhamnosidase treatment on hesperetin conjugate profiles, a further treatment where (3) orange juice fortified with three times the original content of hesperetin-7-O-rutinoside was used. Five hesperetin metabolites (3'-O-glucuronide; 7-O-glucuronide; 5,7-O-diglucuronide; 3',7-O-diglucuronide; 3'-O-sulphate) were present after all treatments (1-3), with the same profile of the conjugates. The present data show that bioavailability of naringenin is increased by conversion from rutinoside to glucoside, but the profile of the conjugates of flavanones formed and excreted in urine is neither affected by the absorption site nor by a 3-fold change in dose.
DOI: 10.1017/S0007114509993679
PubMed: 20100371
Affiliations:
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Le document en format XML
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Nielsen</name></author><author><name sortKey="Rasmussen, Salka E" sort="Rasmussen, Salka E" uniqKey="Rasmussen S" first="Salka E" last="Rasmussen">Salka E. Rasmussen</name></author><author><name sortKey="Cornett, Claus" sort="Cornett, Claus" uniqKey="Cornett C" first="Claus" last="Cornett">Claus Cornett</name></author><author><name sortKey="Barron, Denis" sort="Barron, Denis" uniqKey="Barron D" first="Denis" last="Barron">Denis Barron</name></author><author><name sortKey="Bouisset, Florilene" sort="Bouisset, Florilene" uniqKey="Bouisset F" first="Florilene" last="Bouisset">Florilene Bouisset</name></author><author><name sortKey="Offord, Elizabeth" sort="Offord, Elizabeth" uniqKey="Offord E" first="Elizabeth" last="Offord">Elizabeth Offord</name></author><author><name sortKey="Williamson, Gary" sort="Williamson, Gary" uniqKey="Williamson G" first="Gary" last="Williamson">Gary Williamson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20100371</idno><idno type="pmid">20100371</idno><idno type="doi">10.1017/S0007114509993679</idno><idno type="wicri:Area/PubMed/Corpus">000829</idno><idno type="wicri:Area/PubMed/Curation">000829</idno><idno type="wicri:Area/PubMed/Checkpoint">000829</idno><idno type="wicri:Area/Ncbi/Merge">000B01</idno><idno type="wicri:Area/Ncbi/Curation">000B01</idno><idno type="wicri:Area/Ncbi/Checkpoint">000B01</idno><idno type="wicri:Area/Main/Merge">001B54</idno><idno type="wicri:Area/Main/Curation">001B16</idno><idno type="wicri:Area/Main/Exploration">001B16</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Absorption, conjugation and excretion of the flavanones, naringenin and hesperetin from alpha-rhamnosidase-treated orange juice in human subjects.</title><author><name sortKey="Bredsdorff, Lea" sort="Bredsdorff, Lea" uniqKey="Bredsdorff L" first="Lea" last="Bredsdorff">Lea Bredsdorff</name><affiliation wicri:level="1"><nlm:affiliation>Technical University of Denmark, National Food Institute, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Technical University of Denmark, National Food Institute, Mørkhøj Bygade 19, DK-2860 Søborg</wicri:regionArea><wicri:noRegion>DK-2860 Søborg</wicri:noRegion></affiliation></author><author><name sortKey="Nielsen, Inge Lise F" sort="Nielsen, Inge Lise F" uniqKey="Nielsen I" first="Inge Lise F" last="Nielsen">Inge Lise F. Nielsen</name></author><author><name sortKey="Rasmussen, Salka E" sort="Rasmussen, Salka E" uniqKey="Rasmussen S" first="Salka E" last="Rasmussen">Salka E. Rasmussen</name></author><author><name sortKey="Cornett, Claus" sort="Cornett, Claus" uniqKey="Cornett C" first="Claus" last="Cornett">Claus Cornett</name></author><author><name sortKey="Barron, Denis" sort="Barron, Denis" uniqKey="Barron D" first="Denis" last="Barron">Denis Barron</name></author><author><name sortKey="Bouisset, Florilene" sort="Bouisset, Florilene" uniqKey="Bouisset F" first="Florilene" last="Bouisset">Florilene Bouisset</name></author><author><name sortKey="Offord, Elizabeth" sort="Offord, Elizabeth" uniqKey="Offord E" first="Elizabeth" last="Offord">Elizabeth Offord</name></author><author><name sortKey="Williamson, Gary" sort="Williamson, Gary" uniqKey="Williamson G" first="Gary" last="Williamson">Gary Williamson</name></author></analytic><series><title level="j">The British journal of nutrition</title><idno type="eISSN">1475-2662</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Beverages (analysis)</term><term>Biological Availability</term><term>Citrus sinensis</term><term>Cross-Over Studies</term><term>Disaccharides (pharmacokinetics)</term><term>Double-Blind Method</term><term>Female</term><term>Flavanones (analysis)</term><term>Flavanones (metabolism)</term><term>Flavanones (pharmacokinetics)</term><term>Fruit (chemistry)</term><term>Glucosides (analysis)</term><term>Glucosides (metabolism)</term><term>Glucosides (pharmacokinetics)</term><term>Glycoside Hydrolases (administration & dosage)</term><term>Glycoside Hydrolases (metabolism)</term><term>Hesperidin (metabolism)</term><term>Hesperidin (pharmacokinetics)</term><term>Humans</term><term>Male</term><term>Placebos</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Glycoside Hydrolases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Flavanones</term><term>Glucosides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Flavanones</term><term>Glucosides</term><term>Glycoside Hydrolases</term><term>Hesperidin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Disaccharides</term><term>Flavanones</term><term>Glucosides</term><term>Hesperidin</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Fruit</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Availability</term><term>Citrus sinensis</term><term>Cross-Over Studies</term><term>Double-Blind Method</term><term>Female</term><term>Humans</term><term>Male</term><term>Placebos</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have determined the absorption, conjugation and excretion of naringenin-7-O-rutinoside (narirutin) compared to the corresponding glucoside in an orange juice matrix in human subjects. Healthy volunteers (eight men and eight women), in a double blind, randomised, crossover study, consumed orange juice with (1) natural content of naringenin-7-O-rutinoside; (2) alpha-rhamnosidase-treated to yield naringenin-7-O-glucoside. Blood was sampled at twelve time points and three fractions of urine were collected over 24 h. The area under the plasma-time curve of naringenin from (2) alpha-rhamnosidase-treated orange juice was increased about 4-fold (P < 0.0001), peak plasma concentration (Cmax) was 5.4-fold higher (P < 0.0001) and Tmax was decreased from 311 to 92 min (P = 0.002) compared to untreated orange juice (1), indicating a change in absorption site from the colon to the small intestine. Furthermore, the amount in urine was increased from 7 to 47 % (P < 0.0001) of the dose after consumption of the alpha-rhamnosidase-treated orange juice (2). All urine samples contained both naringenin-7- and -4'-O-glucuronides. In addition, to examine the effect of dose and alpha-rhamnosidase treatment on hesperetin conjugate profiles, a further treatment where (3) orange juice fortified with three times the original content of hesperetin-7-O-rutinoside was used. Five hesperetin metabolites (3'-O-glucuronide; 7-O-glucuronide; 5,7-O-diglucuronide; 3',7-O-diglucuronide; 3'-O-sulphate) were present after all treatments (1-3), with the same profile of the conjugates. The present data show that bioavailability of naringenin is increased by conversion from rutinoside to glucoside, but the profile of the conjugates of flavanones formed and excreted in urine is neither affected by the absorption site nor by a 3-fold change in dose.</div></front></TEI><affiliations><list><country><li>Danemark</li></country></list><tree><noCountry><name sortKey="Barron, Denis" sort="Barron, Denis" uniqKey="Barron D" first="Denis" last="Barron">Denis Barron</name><name sortKey="Bouisset, Florilene" sort="Bouisset, Florilene" uniqKey="Bouisset F" first="Florilene" last="Bouisset">Florilene Bouisset</name><name sortKey="Cornett, Claus" sort="Cornett, Claus" uniqKey="Cornett C" first="Claus" last="Cornett">Claus Cornett</name><name sortKey="Nielsen, Inge Lise F" sort="Nielsen, Inge Lise F" uniqKey="Nielsen I" first="Inge Lise F" last="Nielsen">Inge Lise F. Nielsen</name><name sortKey="Offord, Elizabeth" sort="Offord, Elizabeth" uniqKey="Offord E" first="Elizabeth" last="Offord">Elizabeth Offord</name><name sortKey="Rasmussen, Salka E" sort="Rasmussen, Salka E" uniqKey="Rasmussen S" first="Salka E" last="Rasmussen">Salka E. Rasmussen</name><name sortKey="Williamson, Gary" sort="Williamson, Gary" uniqKey="Williamson G" first="Gary" last="Williamson">Gary Williamson</name></noCountry><country name="Danemark"><noRegion><name sortKey="Bredsdorff, Lea" sort="Bredsdorff, Lea" uniqKey="Bredsdorff L" first="Lea" last="Bredsdorff">Lea Bredsdorff</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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